Non-Viral Methods Direct DNA injection is the simplest method of non-viral transfection. (Wolff JA et al, 1990) Clinical trials have been performed successfully to inject naked DNA plasmids. Trials with naked PCR products have also been done and it had greater success. Many non-viral methods of gene transfer have been yielded by the research efforts. These method includes sonoporation, electroporation, magnetofection, receptor mediated gene transfer and gene gums are being explored.. (Wagner E et al, 1994) Ormasil is also used as a non-viral method. All methods have their own disadvantages and advantages. Injection of Naked DNA For the non-viral transfection it is the simplest method. Clinical trials have been done to carried out intramuscular injection of a naked DNA plasmid that have occurred with some success; though, in comparison to other transfection methods this expression has been very low. (PATIL P.M et al, 2012) Physical Methods to Enhance Delivery ...