Chemical Methods to Enhance Delivery Oligonucleotides In the disease process, for the inactivation of the genes in gene therapy; the use of synthetic oligonucleotides is involved. This is achieved by so many different methods. One strategy uses small RNA molecules called SiRNA to signal the cell to cleave specific unique sequences in the mRNA transcript of the faulty gene. This results in the disruption of translation of the faulty mRNA and consequently expression of the gene. . To disrupt the transcription of the faulty gene, another strategy uses antisense specific to the targeted gene. Lipoplexes and polyplexes For the improved delivery of the new DNA into cell, DNA must be secured from positively charged and damage. Primarily, neutral lipids and anionic were used for the construction of lipoplexes for synthetic vectors. Dendrimers Dendrimer is spherical shaped, highly branched macromolecule. The particle surface may be functionalized in so many ways and so ...